. Author manuscript; available in PMC: 2013 Sep 25.

Published in final edited form as: Biochemistry. 2012 Sep 12;51(38):7488–7495. doi: 10.1021/bi300456f

Table 1.

Octopamine Formed After Met471Cys TβM Inactivation.

Incubation Time (h)^a	[Octopamine] (μM)^b 1 h	[Octopamine] (μM)^b 4 h	[Octopamine] (μM)^b 6 h
0	163.4	350.3	355.2
2	8.07	17.5	20.1
4	4.02	8.28	11.6

The number of hours all reaction components were allowed to react before the addition of 1 mM tyramine.

For each assay, 2.0 μM Met471Cys TβM was incubated with buffer (100 mM KPi, pH 6.0), KCl (100 mM), ascorbate (50 mM), catalase (100 μg/mL), CuSO₄ (10 μM). Following the addition of 1 mM tyramine, aliquots (115 μL) were removed at 5, 10, 15, 30, 60, 120, 240 and 360 min intervals, quenched with 1.5 μL of 70 % HClO₄, and assayed for octopamine formation. Data are shown after 1, 4, and 6 h.