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. 1973 Nov;12(5):969–983. doi: 10.1128/jvi.12.5.969-983.1973

Determination of the Molecular Weight of Animal RNA Viral Genomes by Nuclease Digestions I. Vesicular Stomatitis Virus and Its Defective T Particle

Patricia Repik 1, D H L Bishop 1
PMCID: PMC356726  PMID: 4358168

Abstract

A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T1 to digest viral RNA labeled by 3H-uridine, 3H-cytidine, or 3H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 ± 0.14 × 106 whereas that of the VSV-defective T particle is estimated as 1.23 ± 0.04 × 106.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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