Figure 3.

Gene-Expression Levels Determined by the Luciferase Assay

(A) Schematic of reporter gene constructs. Known regulatory elements (SP1 sites, CAAT box, and TATA box) in the promoter of THBD are schematically represented.

(B) Luciferase activity. Transfection and luciferase assay were performed in three kinds of cells (HUVECs, HEK 293 cells, and COS-7 cells). The pGL3-basic vector was used as a negative control. The luciferase activities are expressed as the ratio of firefly to Renilla luciferase units. The values represent means ± SD of six independent experiments. The black column represents the wild-type construct, and the gray column represents the mutant construct. Error bars represent the SD.