Figure 4.

Heterogeneity Effects of the Nuclease Activity of Altered Forms of DNA2

(A) 0.2 nM WT DNA2 was mixed with 0.2 nM WT or p.Arg284His, p.Lys313Glu, or p.Val723Ile forms of DNA2. The DNA2 mixture was incubated with 0.25 pmol 5′ end ³²P-labeled flap DNA substrates. Reactions were carried out at 37°C for 10, 20, 40, and 60 min.

(B) Quantification of DNA2 cleavage products. Values are means ± SEM of three assays. In each panel, the p value was calculated by a Student’s t test.