Fig. 6.

Lentivirus-mediated delivery of beclin-1 to aged gp120 tg mice increases autophagy markers. Immunohistochemical and confocal analysis 3 months after injection. a Beclin-1 immunoreactivity in non-tg and gp120 tg mice that were injected with LV-control and LV-beclin-1. b Cathepsin-D immunoreactivity in non-tg and gp120 tg mice that were injected with LV-control and LV-beclin-1. c LC3 immunoreactivity in non-tg and gp120 tg mice that were injected with LV-control and LV-beclin-1. d mTor immunoreactivity in non-tg and gp120 tg mice that were injected with LV-control and LV-beclin-1. Semiquantitative levels of e beclin-1, f cathepsin-D, g LC3, and h mTor illustrated as optical density (e, f, and h) or pixel intensity (g). Samples were further stained with DAPI to visualize nuclei (blue). Statistical significance (*p < 0.05, one-way ANOVA, post hoc Fisher’s test) compared to non-tg controls. All panels are from the frontoparietal cortex of 12-month-old mice, a total of n = 20 mice (n = 5 per group) were included for the study