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. 2012 Oct 31;121(6):905–917. doi: 10.1182/blood-2012-03-416503

Figure 2.

Figure 2

Notch progressively depletes HSC activity and numbers over time. (A) Lethally irradiated CD45.1+ congenic mice were reconstituted with 5-FU–treated donor Rag1−/−CD45.2+ BM cells transduced with MigR1 control or activated Notch1 (ΔEGFΔLNRΔP). BM DN T-cell production (Thy-1+CD25+) was measured at 6 weeks and 13 weeks after transplantation. Cells are gated on the CD45.2+GFP+Lineage− cells. (B) Scatter plot analysis of DN T-cell production by MigR1 controls and Notch mice at 6 and 13 weeks after transplantation. (C) The LT-HSC compartment (Lineage−Sca-1+c-Kit+CD34−Flt3−) was measured for donor reconstitution by GFP+ MigR1 control and Notch-activated HSCs at 6 and 13 weeks after transplantation. (D) Scatter plot analysis of donor-derived (GFP+) HSCs (Lineage−Sca-1+c-Kit+CD34−Flt3−) at 6 and 13 weeks after transplantation. Experiments were performed 3 times.