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. 2012 Oct 31;121(6):905–917. doi: 10.1182/blood-2012-03-416503

Figure 3.

Figure 3

HSPCs are transiently driven into cell cycle by Notch signaling. (A) Lethally irradiated mice were reconstituted with 5-FU–treated donor Rag1−/− BM cells transduced with MigR1 control or activated Notch1. At 2 weeks and 6 weeks after transplantation, the mice were injected with BrdU and then killed 16 hours later. (A) The donor-derived BM HSPC compartment (GFP+Lineage−Sca-1+c-Kit+) was stained with 7-AAD and intracellular antibody against BrdU. Cells entering cell cycle are BrDU+ with 2N DNA content by 7-AAD stain. Apoptotic cells are BrdU− and found in the sub2N region of the 7-AAD stain. (B) Bar graph analysis of BrdU+ MigR1 control and Notch HSPCs entering cell cycle at 2 and 6 weeks after transplantation. Three mice per condition.