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. 2012 Oct 31;121(6):905–917. doi: 10.1182/blood-2012-03-416503

Figure 6.

Figure 6

Notch progressively depletes LT-HSC activity and numbers over time in nontransplanted Rosa26-Flox-STOP-Flox-Notch-GFP mice. Mx-cretgRosa26-Flox-STOP-Flox-YFP control mice (“YFP control”) and Mx-cretgRosa26-Flox-STOP-Flox-ICN-GFP (“Notch”) mice were injected with pI-pC to activate YFP control and ICN-GFP, respectively in HSCs. (A) BM DP T-cell production (CD4+CD8+) by the HSC compartment was measured at 4 and 16 weeks after injection in YFP control and Notch mice. (B) Scatter plot analysis of BM DP T-cell production by YFP control and Notch mice at 4 and 16 weeks after injection. (C) The LT-HSC compartment (Lineage−Sca-1+c-Kit+CD150+CD48−) was measured for the donor-derived cells (YFP+ or GFP+) in YFP control and Notch mice at 4 and 16 weeks after injection. (D) Scatter plot analysis of donor-derived (YFP+ or GFP+) LT-HSCs (Lineage−Sca-1+c-Kit+CD150+CD48−) in YFP control and Notch mice at 4 and 16 weeks after injection. One experiment was performed; 3 mice per condition at 4 weeks and 5 mice per condition at 6 weeks.