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. 2012 Dec 28;304(4):L276–L286. doi: 10.1152/ajplung.00299.2012

Fig. 1.

Fig. 1.

Decreased Trek-1 protein expression in A549 cells after short-hairpin RNA (shRNA) transfection. A: a representative Western blot experiment. B: densitometry analysis of 3 Western blot experiments after normalization of band intensities to GAPDH. C: decreased Trek-1 immunofluorescence by confocal microscopy (Trek-1 in green; n = 3) can be seen. Nuclei are stained with DRAQ5 (blue; magnification ×60). *P < 0.05 compared with control.