Skip to main content
. 2012 Dec 28;304(4):L276–L286. doi: 10.1152/ajplung.00299.2012

Fig. 4.

Fig. 4.

Expression and phosphorylation of p38 kinase in MLE-12 cells using Western blot. Total p38 protein expression was unchanged between control and Trek-1-deficient cells at baseline and after 6 h of TNF-α (5 ng/ml) stimulation, but p38 phosphorylation was decreased in Trek-1-deficient cells at 6 h compared with cells transfected with a scrambled control (A). Densitometry analysis of 3 experiments depicting phosphorylated p38 levels normalized to total p38 protein confirms a decrease in p38 phosphorylation levels in Trek-1-deficient cells at 6 h (B, n = 3, P < 0.05; *compared with control cells at 0 min, #compared with Trek-1-deficient cells at 0 min, xcontrol cells at 6 h compared with Trek-1-deficient cells at 6 h). Treatment of cells with the p38 inhibitor SB-202190 before TNF-α stimulation deceased IL-6 release in control but not in Trek-1-deficient cells (C; n = 5, ^P < 0.05).