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After 6 h of TNF-α treatment, calphostin C (0.2 μM) inhibited TNF-α-induced IL-6 release from control but not Trek-1-deficient cells (A), whereas after 24 h of TNF-α treatment, calphostin C inhibited IL-6 release from both control and Trek-1-deficient cells (B; #P < 0.05, n = 3–5). *P < 0.05 compared with control.
