Fig. 6.
Trek-1 deficiency alters phosphorylation of PKC isoforms at baseline and after TNF-α stimulation in MLE-12 cells. A–F: representative Western blot experiments for the phosphorylated isoforms PKC/PKDμ (Ser744/748), PKC/PKDμ (Ser916), PKCδ, PKCα/βII, PKCζ/γ, and PKCθ. F also includes a blot showing unchanged total PKCθ expression in control and Trek-1-deficient cells. G–L: densitometry analyses of 5 experiments/isoform. Band densities were normalized to GAPDH (P < 0.05, *compared with scrambled control at each time point, #compared with scrambled control at 0 min). M: a decrease in IL-6 release from control but not Trek-1-deficient A549 cells in the presence of a myristolated PKCθ pseudosubstrate inhibitor (*P < 0.05, n = 3) can be seen. Baseline IL-6 secretion from control or Trek-1-deficient cells was not affected by this PKCθ inhibitor.