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. 2012 Dec 28;304(4):L250–L263. doi: 10.1152/ajplung.00278.2012

Fig. 1.

Fig. 1.

Receptor for advanced glycation end products (RAGE) expression in whole lung and lung microvascular endothelial cells. A: monoclonal antibody detects a 45-kDa RAGE isoform in human lung. B: polyclonal antibody raised to the NH2-terminus confirms the presence of the 45-kDa isoform and detects additional 51- and 64-kDa isoforms. C: polyclonal antibody raised to the COOH-terminus detects all three RAGE isoforms in human lung. D: monoclonal antibody only detects RAGE in immortalized lung endothelial cells after a long exposure (20 min). IMVEC-L, immortalized lung microvascular endothelial cells. F: polyclonal antibody raised to the COOH-terminus detects RAGE in immortalized lung endothelial cells.