Fig. 6.

RAGE expression on murine lung tissue. A: immunoblotting of lung tissue from C57Bl/6J and RAGE knockout (KO) mice. Immunofluorescence of RAGE (B), platelet endothelial cell adhesion molecule (PECAM) KO (C), and wild-type (WT) lungs (B and C) reveals that antibodies are specific. D: localization of RAGE on major lung structures (green, PECAM; red, RAGE). Only small amounts of EC RAGE are visualized at baseline (arrowheads). E: proximity ligation assay (PLA) demonstrates RAGE and PECAM colocalization in murine lung tissue. PLA on lung sections from RAGE KO (a), PECAM KO (b), or WT (c) mice. Lung sections from WT mice display a positive signal.