Fig. 7.

The RAGE ligand N^ε-carboxymethyl lysine (N^ε-CML) is detectable on murine RBCs, and endothelial RAGE expression is increased following RBC transfusion. A: there is a dose-dependent increase in detectable N^ε-CML on intact murine RBCs (P = 0.004). B: N^ε-CML on 1- and 14-day-old murine RBCs (P = 0.020). PECAM and RAGE expression following 24 h of PBS transfusion (C) or RBC transfusion (D), magnification ×20. E: ×100 magnification of lung from RBC-transfused animal; white arrows denote RAGE and PECAM-labeled endothelial cells. *P < 0.05.