Fig. 8.

Endothelial RAGE and PECAM expression in naïve and RBC-transfused mice. A: RBC-transfused mice were perfused with IgG control antibodies. B: PBS-treated mice perfused with α-PECAM and α-RAGE antibodies. C: RBC-transfused mice perfused with α-PECAM and α-RAGE antibodies. D: quantification of endothelial RAGE expression from 3 random fields using Image J software. Endothelial RAGE expression is increased 4 h following RBC transfusion, P < 0.001. Representative images from 1 experiment; 3 independent experiments were conducted. Original magnification ×40. *P < 0.05.