Fig. 2.

ADAM15 is upregulated in the lung tissue of mice receiving LPS. A: Western blot analysis of ADAM15 expression in mouse lung tissue. Bottom, representative blot from C57/BL6 WT mice 24 h after intraperitoneal injection of LPS (10 mg/kg body wt) or an equivalent volume of PBS. Top, quantification (n = 4; *P < 0.05 vs. PBS treated). B: immunofluorescence microscopy shows increased ADAM15 staining in LPS-treated compared with PBS-treated lungs. Lung sections were labeled with ADAM15 antibody, followed by phycoerythrin-conjugated second antibody. C: Western blotting and densitometry quantification (top, n = 3, *P < 0.01) of ADAM15 expressed by in vitro cultured endothelial cells with or without LPS stimulation (12 h.). D: immunocytochemistry of untreated (left) of LPS-treated cells (as in C). Green, ADAM15; red, VE-cadherin; blue, nuclei.