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. 2012 Nov 16;304(3):L135–L142. doi: 10.1152/ajplung.00133.2012

Fig. 4.

Fig. 4.

ADAM15 expression is detectable in endothelial cells but not in neutrophils. A: flow cytometric evidence of cell identity and purity. Left, primary lung microvascular EC (MLEC) from Adam15+/+ or Adam15−/− or mice labeled with an anti-CD31 antibody or IgG isotype control. Right, neutrophils from mouse bone marrow (mPMN) are labeled with an anti-Ly-6G antibody, and granulocytes are gated. Isotype control is also shown. The data represent 3 independent experiments from different mice. B: ADAM15 expression in MLEC and mPMN. Shown are representative blots from 3 independent experiments demonstrating abundant ADAM15 in Adam15+/+ EC but not in neutrophils.