Fig. 6.

Endothelial ADAM15 mediates neutrophil transendothelial migration. A: mPMN transmigration across Adam15^−/− MLECs, especially in the presence of fMLP (10 nM), is significantly attenuated (n = 3; *P < 0.05 vs. Adam15^+/+ without fMLP; #P < 0.05 vs. Adam15^+/+ with fMLP). B: rescue expression of ADAM15 in Adam15^−/− MLECs is confirmed with Western blotting (left) and flow cytometry (right). C: rescue expression of ADAM15 in Adam15^−/− MLECs increased neutrophil transendothelial migration. Adam15^−/− MLECs were transfected with pcDNA/Adam15 or mock. At 72 h posttransmigration, fMLP-induced neutrophil transendothelial migration was assayed as above (n = 3, *P < 0.05 vs. mock transfected).