Fig. 5.

nAChRs are absent in mature granule cells (GCs). A: a field of GCs loaded with fura 2-AM. B: response from these cells to a 5-s puff of ACh/AT. Only 3 cells responded in this field. The cell with the largest response is circled in A. Two cells, marked with arrows in A, showed smaller responses. Traces at right are 15 min after application of DNQX/APV. Responses from the small fraction of GCs that respond to ACh/AT can be completely blocked by DNQX/APV, unlike that seen in NPCs (inset C). ***P < 0.001.