Figure 3.

Altered cell types in 1, 2, 4, and 10 month Nrl^−/− retina. A, Ganglion cells were immunolabeled with anti-Brn3a (red) and anti NF-L (green) and showed presence of displaced ganglion cells in the INL of young animal (1 and 2 months of age). These were not present in 4- and 10-month-old animals. B, Müller cells, immunolabeled with anti-Sox9 antibody (green), migrated first around the remaining pseudorosettes (at 4 months), and then in the entire onl (10 months). C, GFAP immunolabeling (red) showed strong activation of Müller cells at 2, 4, and 10 months of age.