Figure 8.

Lipid peroxidation in Nrl^−/− retina. A, Immunolabeling of acrolein (green), a toxic product formed from lipid peroxidation and used as a marker of oxidative stress, was increased at 2 and 4 months in Nrl^−/− mice, relative to 1 and 10 months. Scale bar, 20 μm. B, Thiobarbituric acid-reactive substances assay was used to assess lipid peroxidation in retinal lysates of 1-, 2-, 4-, 10-month-old Nrl^−/− mice and of 1- and 10-month-old WT mice. The assay shows a significant increase of MDA in 4-month-old Nrl^−/− mouse retina. Error bars indicate ±SD. Standard t test was used to determine significance. *p < 0.05.