Figure 1.

Changes in Cx36 protein expression during development and following ischemia in vitro. Data from Western blot experiments in neuronal somatosensory cortical cultures are presented. A, Developmental changes in Cx36 expression. B, OGD-induced changes in Cx36 expression in mature cultures [day in vitro 25 (DIV25)]. Duration of OGD was 30 min. A representative blot (above) and statistical data (below) are shown. In both graphs, optical density signals are normalized relative to tubulin and compared with the control (day in vitro 1 in A; pre-OGD condition in B; set at 1.0). Statistical analysis was as follows: ANOVA with post hoc Tukey relative to the control; n = 6 (A) and 5–11 (B) per group; data are shown as mean ± SEM. NS, Nonsignificant.