Figure 10.

Group II mGluRs regulate increase in Cx36 expression and neuronal death during different types of neuronal injury: hypoosmotic shock (HOS). Data from Western blot (A, B), RT-qPCR (C), and MTT (D) experiments in wild-type mouse mature neuronal somatosensory cortical cultures are presented. Experimental conditions, drug concentrations, and analyses are the same as in OGD experiments shown in Figures 1B, 2C, 8A, and 9A; however, because cultures were subjected to HOS for 60 min (see Materials and Methods), the drug treatments were 30 min longer than during OGD. Statistical analysis was as follows: ANOVA with post hoc Tukey; shown relative to control (i) and nontreated plus HOS (ii); n = 5–10 (A), 4–7 (B), 5 (C), and 10 (D) per group; mean ± SEM.