Figure 11.

Group II mGluRs regulate increase in Cx36 expression and neuronal death during different types of neuronal injury: hydrostatic pressure injury (HPI). Data from Western blot (A, B), RT-qPCR (C), and MTT (D) experiments in wild-type mouse mature neuronal somatosensory cortical cultures are presented. Experimental conditions, drug concentrations, and analyses are the same as in ischemic experiments shown in Figures 1B, 2C, 8A, and 9A; however, because cultures were subjected to HPI for 60 min (see Materials and Methods), the drug treatments were 30 min longer than during OGD. Statistical analysis: ANOVA with post hoc Tukey; shown relative to control (i) and nontreated plus HPI (ii); n = 4–12 (A), 5–7 (B), 5 (C), and 6–8 (D) per group; data are shown as mean ± SEM.