Figure 9.

Inactivation of group II mGluRs and neuronal gap junctions both reduce ischemic neuronal death. A, B, Statistical data from MTT experiments in wild-type (A) and Cx36 knock-out (B) mature neuronal cortical cultures are shown. Statistical analysis was as follows: ANOVA with post hoc Tukey; shown relative to nontreated plus OGD group; ***p < 0.001; n = 6–12 per group. C–H, Statistical data (C) and representative images (D–H) from Fluoro-Jade B staining experiments in adult mice are shown. All images are taken at bregma −1.7 mm (i.e., at the center of ischemic injury). Images in D–F are taken from the same brain section and the regions that are boxed in D and E are shown at a higher magnification as E and F, respectively. Saline and LY341495 injections were done in triplicate: immediately before, immediately after, and 24 h after the induction of ischemia (in doses as in Fig. 4). In C, the number of stained pixels in the ischemic cortical region is analyzed: ANOVA; statistical difference is shown relative to the saline-treated WT mice (***p < 0.001) and between saline- and LY341495-treated Cx36 knock-out mice (NS, nonsignificant); n = 4–7 mice per group; data are shown as mean ± SEM. Hipp, Hippocampus; SSp, primary somatosensory cortex; Thal, thalamus.