Table 1.

Summary of different approached undertaken for mineral biofortification of rice.

Gene	Promoter	Cultivar	Fold increase in Fe/Zn	Reference
AtNAS1+, Pvferritin+, Afphytase	CaMV 35S, Glb-1, Glb-1	Japonica cv. Taipei 309	6.3/1.61	Wirth et al. (2009)
HvNAS1	Actin	Japonica cv. Tsukinohikari	3.4/2.32	Masuda et al. (2009)
HvNAS1, HVNAS1+HvNAAT, IDS3	Genomic fragments	Japonica cv. Tsukinohikari	1.0/1.02, 1.1/1.1, 1.4/1.3	Masuda et al. (2008)
OsNAS1, OsNAS2, OsNAS3	CaMV 35S	Japonica cv. Nipponbare	2.2/1.4, 4.2/2.2, 2.2/1.4	Johnson et al. (2011)
OsNAS2	Activation tagging	Japonica cv. Dongjin	3/2.7	Lee et al. (2011, 2012b)
OsNAS3	Activation tagging	Japonica cv. Dongjin	/2.2	Lee et al. (2009b)
OsNAS1	GluB1	Japonica cv. Xiushui 110	1.0/1.3	Zheng et al. (2010)
SoyferH-1	Glu-B1	Japonica cv. Kitaake	3.0	Goto et al. (1999)
PvFerritin+ rgMT	Glb-1	Japonica cv. Taipei 309	2.03	Lucca et al. (2001)
SoyFer	Glu-B1	Indica cv. IR68144	3.7/1.4	Vasconcelos et al. (2003)
SoyFer	Glu-B1; Glb-1	Japonica cv. Kitaake	3.0/1.1	Qu et al. (2005)
OsFer2	OsGluA2	Basmati rice (Indica cv. Pusa-Sugandh II)	2.1/1.4	Paul et al. (2012)
TOM1	CaMV 35S	Japonica cv. Tsukinohikari	1.2/1.6	Nozoye et al. (2011)
OsYSL2	OsSUT1	Japonica cv. Tsukinohikari	4.4	Ishimaru et al. (2010)
OsIRT1	Ubi	Japonica cv. Dongjin	1.1/1.13	Lee and An (2009)
OsYSL2+, SoyFerH2+, HvNAS1	OsSUT1, Glb-1, Glb-1. Glu-B1, Act	Japonica cv. Tsukinohikari	6 or 44/1.6	Masuda et al. (2012)

¹Mn concentration also increased by two fold.

²Plants were tested in isolated filed.

³Concentration in brown rice.

⁴Six times in T2 seeds while 4.2 times in T3 seeds.