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. 2012 Nov 30;32(3):354–368. doi: 10.1038/emboj.2012.315

Figure 7.

Figure 7

C-di-GMP directly binds to both PgaC and PgaD. Membranes containing wild-type and mutant forms of PgaC–3 × Flag and/or PgaD–3 × Flag were UV crosslinked in the presence of [32P]c-di-GMP and with (black bars) or without excess c-di-GMP (grey bars). Relative PgaC (upper graph) and PgaD (lower graph) autoradiography band intensities are shown as an average of two independent experiments with standard deviations.