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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nanomedicine (Lond). 2012 Mar 6;7(6):877–888. doi: 10.2217/nnm.11.185

Figure 4. Quantification of vimentin and cowpea mosaic virus in lesion versus nonlesion tissue.

Figure 4

Analysis of vimentin and CPMV in tissue sections using ImageJ. (A) Mean fluorescence of vimentin staining in the endothelium for C57Bl/6J control mice on normal chow, and lesion and nonlesion areas of LDLR−/− mice on a high-fat diet for 12 weeks. Mean fluorescence of vimentin was statistically significant between control C57Bl6/J tissue and LDLR−/− lesion (p < 0.01) and not statistically significant between LDLR−/− nonlesion and lesion tissue. (B) Mean fluorescence of CPMV in endothelium for control C57BL6/J mice on normal chow and lesion and nonlesion areas of LDLR−/− mice on high-fat diet for 20 weeks (p < 0.0001, between LDLR−/− lesion and both LDLR−/− nonlesion and control). (C) Percent of endothelial area positive for CPMV particle fluorescence in control and atherosclerotic mouse aorta after 12 and 20 weeks on a high-fat diet using the color threshold plugin for ImageJ. LDLR−/− lesion tissue compared with both nonlesion LDLR−/− tissue and control tissue was statistically significant (p < 0.001). (D) Percent total lesion area positive for CPMV particle fluorescence using color threshold plugin for ImageJ.

CPMV: Cowpea mosaic virus; LDLR−/−: Low-density lipoprotein receptor knockout; MFI: Median fluorescence intensity.