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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nanomedicine (Lond). 2012 Mar 6;7(6):877–888. doi: 10.2217/nnm.11.185

Figure 5. Reduced cowpea mosaic virus interaction with differentiated macrophages in vitro.

Figure 5

(A) Confocal image of lipid staining of untreated RAW macrophages with ORO (red). (B) Confocal image of lipid staining of oxLDL-treated RAW macrophages with ORO (red). (C) Confocal image of CPMV uptake by untreated RAW macrophages (green). (D) Confocal image of CPMV uptake by oxLDL-treated RAW macrophages (green). (E) Confocal image of lipid staining of untreated TSPM with ORO (red). (F) Confocal image of lipid staining of oxLDL treated TSPM with ORO (red). (G) Confocal image of CPMV uptake by untreated TSPM (green). (H) Confocal image of CPMV uptake by oxLDL-treated TSPM (green). All confocal images were obtained at 60× and the nucleus has been stained with 4′,6-diamidino-2-phenylindole (blue). (I) FACS analysis of CPMV uptake in RAW macrophages with and without oxLDL treatment. (J) FACS analysis of number of RAW macrophages stained positive for surface vimentin with oxLDL treatment for 24 h (p = 0.22, not significant) and 72 h (p = 0.09, not significant).

CPMV: Cowpea mosaic virus; ORO: Oil Red O; oxLDL: Oxidized low-density lipoprotein;

TSPM: Thioglycolate-stimulated mouse peritoneal macrophages.