FIGURE 6.

Expression levels of mMCPs, CPA, and Hdc mRNAs in WT, HS6ST-2^−/−, and HS6ST-1^−/−/HS6ST-2^−/−-FSMCs. Total RNA was isolated from the FSMCs of WT and HS6ST-2^−/− littermates (A and B) or HS6ST-2^−/− and HS6ST-1^−/−/HS6ST-2^−/− littermates (C and D). A and C, expression of each mRNA was measured by semi-quantitative RT-PCR using primer pairs as described under “Experimental Procedures.” The expression level of β-actin was used as a control. The mRNA expression was compared between WT and HS6ST-2^−/− FSMCs (A) or between HS6ST-2^−/− and HS6ST-1^−/−/HS6ST-2^−/−-FSMCs (C). B and D, mRNA expression was quantitatively measured using SYBR Green according to the manufacturer's protocol, and expression levels were normalized to β-actin expression as described under “Experimental Procedures.” The mRNA expression was compared between WT and HS6ST-2^−/−-FSMCs (B) or between HS6ST-2^−/− and HS6ST-1^−/−/HS6ST-2^−/−-FSMCs (D). Relative mRNA expression values were obtained from two independent experiments that were performed in triplicate, and error bars represent the standard deviation.