FIGURE 1.

Identification of high molecular weight proteins in chemically demembranated flight muscle samples. A, skinned protein samples prepared from w¹¹¹⁸ (lanes 1 and 3) and Mhc¹⁰ (lanes 2 and 4) flight muscles were resolved in a 4–20% polyacrylamide gel and stained with either Coomassie G-250 (left panel) or anti-Z(210) antibody (right panel). Note the bands of apparent mass ∼240 and ∼260 kDa that are revealed in the absence of MHC (arrowheads). B, results of the LC-MS/MS analysis. The protein bands of ∼240 and 260 kDa (arrowheads in panel A) were excised from the gel and subjected to LC-MS/MS analysis. Probability indicates the level of certainty that the named polypeptide corresponds to a component of the sample analyzed.