FIGURE 6.

CNTF secreted by GSNO treated astrocytes enhances OL differentiation. Immature OLs (2000 cells/cm²) plated in glass slide chambers or 6-well culture plates were cultured for 96 h in culture supernatants (astrocyte conditioned media; 100%) of astrocytes treated with GSNO (GSNO-ACM) or vehicle (Control-ACM). A, a representative phase contrast micrograph (n = 5) of immature OLs cultured in control-ACM or GSNO-ACM in the presence or absence of CNTF neutralizing antibodies (α-CNTF-Abs) depicts OL morphology photographed by light microscopy at magnification ×200. B, the composite mean ± S.E. of four experiments depicts the number of processes per cell determined by counting of the phase contrast micrographs. C, the composite mean ± S.E. of four experiments depicts the length of process per cell measured in the phase contrast micrographs. D, the composite mean ± S.E. of four experiments depicts the ratio of MBP to β-actin mRNA transcripts in treated immature OLs. Statistical significance as indicated. *, p < 0.05; **, p < 0.01; ***, p < 0.001.