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. 2012 Dec 21;288(6):3831–3843. doi: 10.1074/jbc.M112.405654

FIGURE 7.

FIGURE 7.

Endogenously produced NO mimics GSNO effect in astrocytes. Cortical astrocytes were plated in 6-well cell culture plates as detailed in the Fig. 1 legend. A and B, the composite mean ± S.E. of three experiments depicts the ratio of CNTF and GFAP to β-actin mRNA transcripts in astrocytes treated with TNF-α (15 ng/ml) plus IFN-γ (25 ng/ml) in the presence of apocynin or vehicle as well as in transiently transfected astrocytes with p47-phox or control (scramble) siRNAs and treated with L-NAME for 24 h. C, the composite mean ± S.E. of three experiments depicts the ratio of MBP to β-actin mRNA transcripts in immature OLs plated as detailed in Fig. 6 legend and cultured for 96 h in culture supernatants (astrocyte-conditioned media; 50%) of cytokine-treated astrocytes (A and B). D, the composite mean ± S.E. of three experiments depicts nitrite levels in the culture supernatants of cytokine treated astrocytes for 24 h. Statistical significance as indicated. *, p < 0.05; **, p < 0.01; ***, p < 0.001. NS, not significant.