Skip to main content
. 2012 Dec 19;288(6):3844–3857. doi: 10.1074/jbc.M112.426338

FIGURE 4.

FIGURE 4.

HIF1α suppresses the expression of adiponectin through the SOCS3-STAT3 pathway in vitro. A, qPCR and Western blot analysis of the knockdown efficacy by Socs3 siRNA (SiSOCS3). Adipocytes were transfected with siRNA for 24 h. B, effect of knockdown of Socs3 on the expression of adiponectin mRNA in adipocytes. Adipocytes were transfected with siRNA. After 24 h, the cells were incubated for 16 h with 200 μm CoCl2. C, Western blot analysis of the tyrosine phosphorylation of STAT3 in adipocytes. SiHIF1α, HIF1α siRNA. D, effect of the STAT3 inhibitor NSC 74859 (100 μm) on the expression of adiponectin mRNA in adipocytes under hypoxic conditions with CoCl2. For qPCR analysis, expression was normalized to β-actin. Data are means ± S.D. **, p < 0.01 compared with the control (scrambled siRNA); #, p < 0.05; ##, p < 0.01 compared with scrambled siRNA + CoCl2 or HIF1α siRNA treatment.