FIGURE 5.

Complement-induced PGE₂ production in GECs that stably express M1 GFP-iPLA₂γ WT is mediated by ERK and p38. A, GECs were incubated with antibody and complement (as in Fig. 4) in the presence of inhibitors of p38 (SB203580; 10 μm), MEK1 (PD98059; 50 μm), or JNK (SP600125; 10 μm). Complement-induced PGE₂ production was reduced significantly by the p38 and MEK1 inhibitors. *, p < 0.0001 NS versus HIS (vehicle); **p < 0.01 SB203580 versus vehicle (NS); +, p < 0.001 PD98059 versus vehicle (NS), six experiments. B, GECs were incubated with antibody and complement in the presence of inhibitors of p38 (FR167653; 10 μm) and MEK1 (U0126; 50 μm). Complement-induced PGE₂ production was reduced significantly by both drugs. *, p < 0.0001 NS versus HIS (vehicle); **, p < 0.001 FR167653 versus vehicle (NS); +, p < 0.0001 U0126 versus vehicle (NS), four experiments.