TABLE 1.
PCR primers employed to construct iPLA2γ mutants
Bold letters denote base substitutions encoding for mutated amino acids.
| GFP-iPLA2γ | Primer | Primer sequence (5′ to 3′) |
|---|---|---|
| GFP-iPLA2γ S271A | F1 | CCGGAGCTCCTGCAGTCGACATGTCTATTAATCT |
| R2 | CGCAGAAGGAGCTGTAGGCTTG | |
| F2 | CAAGCCTACAGCTCCTTCTGCG | |
| R1 | CCTTGTTCCTCCACCATCAA | |
| GFP-iPLA2γ S168A | F1 | CCGGAGCTCCTGCAGTCGACATGTCTATTAATCT |
| R3 | GGAAAAGGAGCCTTTTCTGCTGAT | |
| F3 | GCAGAAAAGGCTCCTTTTCCAGAA | |
| GFP-iPLA2γ S511A/S515A | R1 | CCTTGTTCCTCCACCATCAA |
| F1 | GCGAGAAAAGATTATCGCAAG | |
| R4 | TTGTGCAAATACATCTGCTCCTAATTTTC | |
| F4 | AATTAGGAGCAGATGTATTTGCACAAAATGT | |
| R1 | GGGGTTTCTTGCTGTTTCAA | |
| M4 GFP-iPLA2γ | M4-F1 | CCGGAGCTCAAATGAAATGTCTCAACAAAAGGAAAATG |
| M4-R1 | CCTTGTTCCTCCACCATCAA |