TABLE 2.

Effect of the MNK1 inhibitor, CGP57380, on PGE₂ production

COS-1 cells were co-transfected with M1 GFP-iPLA₂γ WT and COX1. After 24 h some cells were treated with CGP57380 (20 μm) for 16 h. Then cells were incubated with EGF + ionomycin (see the legend to Fig. 8) for 40 min. Stimulated PGE₂ production was inhibited by CGP57380. Basal PGE₂ release (untransfected, untreated cells) was 51 pg/ml.

Transfection/treatment	PGE2 fraction
Untransfected, untreated	0.13 ± 0.02
Untransfected + CGP57380	0.17 ± 0.04
GFP-iPLA2γ + COX, untreated	0.65 ± 0.04
GFP-iPLA2γ + COX, EGF + ionomycin	0.91 ± 0.04
GFP-iPLA2γ + COX, EGF + ionomycin + CGP57380	0.56 ± 0.07a

^a p < 0.001 EGF + ionomycin + CGP57380 vs. EGF + ionomycin, three experiments.