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. 2012 Dec 18;288(6):3886–3896. doi: 10.1074/jbc.M112.415679

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FIGURE 4. — Mapping the HTE-binding site on MTB Ag85. A, schematic representation of the truncated M. tuberculosis Ag85B constructs used in this study, including M. tuberculosis Ag85B-N (residues 41–133), M. tuberculosis Ag85B-Ctr1 (residues 134–198), M. tuberculosis Ag85B-Ctr2 (residues 199–253), and M. tuberculosis Ag85B-C (residues 254–325). B, comparison of HTE binding affinity of truncated M. tuberculosis Ag85B proteins. Full-length and truncated M. tuberculosis Ag85B (0.2 μm) was added to microtiter plate wells containing recombinant GST-tagged HTE or BSA (0.2 μg/well). Bound proteins were detected by ELISA.