TABLE 1.
Strain or plasmid | Genotype or characteristic | Sources |
---|---|---|
M. tuberculosis (MTB) H37Ra | Wild type MTB strain | ATCC |
E. coli strains | ||
DH5α | FΦ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK+) supE44 thi−1 gyrA96 relA1λ | Invitrogen |
BL21 | F−, ompT, hsdSB (rB−, mB−), dcm, gal, λ(DE3) | Promega |
Plasmids | ||
pET-32 Xa/Lic | Ampr, Thioredoxin tag, His tag, and S tag protein expression vector | Merck |
pGEX4T-2 | Ampr, GST tag protein expression vector | GE Healthcare |