FIGURE 2.
Histological analysis of Yap1-deficient mice. A–G, ventricles from adult mice were fixed, sectioned, and stained. A, shown is hematoxylin and eosin staining demonstrating disorganized cardiomyocytes and increased cell infiltration in YapF/FCre hearts. B and C, Masson's Trichrome staining revealed a significant increase in interstitial fibrosis in YapF/FCre hearts. D and E, TUNEL shows a significant increase in apoptotic cardiomyocytes in YapF/FCre hearts. Arrows indicate TUNEL-positive nuclei. F and G, wheat germ agglutinin (WGA) staining reveals increased myocyte CSA in YapF/FCre hearts. H, shown is the estimated number of cardiomyocytes present in the hearts of Yap1 mutant and control mice. Values are the means ± S.E. *, p < 0.05. N.S. = not significant. Scale bars, 100 μm.