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. 2012 Dec 30;288(6):3977–3988. doi: 10.1074/jbc.M112.436311

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FIGURE 4. — Yap1-induced cardiomyocyte hypertrophy is Akt-independent. A, cardiomyocytes were transduced with LacZ or Yap1 adenovirus and treated with LY294002 (10 μm), PD98059 (10 μm), or vehicle and stained to detect troponin T. Scale bars, 10 μm. B, quantification of cardiomyocyte surface area is shown. Values are the means ± S.E. *, p < 0.05. N.S. = not significant. C, cardiomyocytes were transduced with LacZ or Yap1 adenovirus, and quantitative real-time-PCR was performed to detect ANF (Nppa), brain natriuretic protein (Nppb), and β-MHC (Myh7) gene expression. Results were normalized to Gapdh. *, p < 0.05. D, cardiomyocytes were transfected with LacZ or Yap1 in combination with ANF-luc reporter plasmid. Cells were treated with LY294002 (10 μm), Akt inhibitor V (triciribine; 10 μm), PD98059 (10 μm), or vehicle control, and luciferase activity was determined. Values are the means ± S.E. *, p < 0.05 versus LacZ + vehicle. N.S. = not significant.