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H2O2 stress activates JNK signaling in rat H9c2 cardiomyocyte-like cells. JNK signaling was evaluated by Western blot following 1, 2, 4, 6, and 8 h of treatment with 25 μm H2O2. Proteins (30 μg) were resolved by SDS-PAGE, and antibodies for A, JNK; B, phospho-JNK; C, c-jun; and D, phospho-c-jun were used to detect proteins of interest. (E) α-Tubulin was used as a loading control.
