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. 2012 Dec 20;288(6):4000–4011. doi: 10.1074/jbc.M112.406777

FIGURE 7.

FIGURE 7.

The selective JNK inhibitor SR-3306 decreases apoptosis and CPK and CK-MB levels in rat hearts during I/R injury in vivo. SR-3306 was given first as a bolus dose 5 min prior to onset of reperfusion and then administered by constant infusion at a rate of 18 μg/kg/min during the reperfusion phase to yield a final total dose of 5 mg/kg. A–F, DAPI- and TUNEL-stained sections from two representative sections of the heart comparing sham-treated animals with a vehicle-treated I/R group of animals and a 5 mg/kg SR-3306-treated I/R group of animals. Total number of nuclei (blue) and TUNEL positive cells (green) are shown for each representative section of the heart. G, percent TUNEL positive nuclei determined by averaging the counts from 30 random images from each heart. The percent TUNEL positive nuclei was defined as the number of TUNEL positive nuclei divided by the total number of nuclei. H, CPK activity given in units/liter for naive rats, rats exposed to I/R injury for 2 h, and rats treated with 5 mg/kg SR-3306. I, CK-MB activity given in units/liter for naive rats, rats exposed to I/R injury for 2 h, and rats treated with 5 mg/kg SR-3306.