FIGURE 5.
Stable silencing of KLF4 attenuates the effect of PPARγ agonists on the modulation of KLF4 target genes. A–C, RNA was extracted from HCT116 cells stably silenced with KLF4 (sh-KLF4) or control (sh-Ctr) shRNA and treated for 24 h with DMSO (control (Ctr)) or TGZ (10 μm). KLF4, p21Waf1, and p27Kip1 expression was measured by real-time qPCR and normalized to GAPDH. Results are the mean ± S.D. of three different experiments. *, p < 0.05; ***, p < 0.001. D, HCT116 cells stably silenced with control or KLF4 shRNA were grown in the absence of serum for 18 h and then incubated with DMSO (−) or TGZ (10 μm; +) for 48 h. The expression of KLF4, p21, and p27 was determined by Western blotting. β-Actin served as an internal control.