FIGURE 6.

Ablation of KLF4 expression reduces PPARγ agonist-mediated G₁/S arrest and anti-proliferation effects in HCT116 cells. A, HCT116 cells stably silenced with control (sh-ctr) or KLF4 (sh-KLF4) shRNA were incubated with DMSO (control) or TGZ (10 μm) for the indicated times, and cell growth inhibition was detected using MTS assay. Values were the mean ± S.D. of absorbance at 570 nm for three independent experiments. B, HCT116 cells stably silenced with control or KLF4 shRNA were left untreated (control (Ctr)) or treated with 10 μm TGZ for 48 h and then analyzed by BrdU incorporation using a BrdU cell proliferation ELISA kit. Similar results were observed in a triplicate analysis. *, p < 0.05. C, cell cycle analysis was conducted at 48 h post-TGZ treatment in control and KLF4 shRNA-silenced HCT116 cells. The percentage of the G₁ phase cells was determined and is shown in a graph. Similar results were observed in a triplicate analysis. *, p < 0.05.