Skip to main content
. 2012 Dec 29;288(6):4128–4134. doi: 10.1074/jbc.M112.440933

FIGURE 5.

FIGURE 5.

KCNB1 oligomerization activates c-Src tyrosine kinase. A, percentage of N2A cells expressing wild-type KCNB1 positive to annexin V in the absence/presence of PP2 in the indicated concentrations. Cells were preincubated for 30 min in DMEM containing PP2. n = 8 experiments. **, p < 0.01. B, as in A in cells transfected with C73A. n = 5 experiments. C, percentage of N2A cells expressing wild-type KCNB1 positive to annexin V in the absence/presence of caspase 8 inhibitor IETD-CHO in the indicated concentrations. Cells were preincubated for 15 min in DMEM containing IETD-CHO. n = 4 experiments. *, p < 0.05. D, percentage of N2A cells cotransfected with wild-type KCNB1 and the indicated c-Src variants positive to annexin V. n = 9 experiments. *, p < 0.05. In all experiments, cells were oxidized by 5-min exposure to 25 μm dTDP. Annexin V staining was performed 6 h post-oxidation.