FIGURE 6.

KCNB1 oligomerization results in JNK activation. A, percentage of N2A cells expressing wild-type KCNB1 positive to annexin V in the absence/presence of specific JNK inhibitor SP600125 in the indicated concentrations. Cells were preincubated for 15 min in DMEM containing SP600125. Cells were oxidized by 5-min exposure to 25 μm dTDP. Annexin V staining was performed 6 h post-oxidation. n = 7 experiments. *, p < 0.05. B, normalized number of N2A cells expressing DCF fluorescence in the absence/presence of PP2 at the indicated conditions. Cells were preincubated for 30 min in DMEM containing PP2. Cells were oxidized by 5-min exposure to 1 mm H₂O₂. DCF-fluorescent cells were counted 3 h post-oxidation. n = 3–4 experiments with cells transfected with wild-type and C73A, respectively.