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. 2012 Dec 12;288(6):4148–4157. doi: 10.1074/jbc.M112.436741

FIGURE 2.

FIGURE 2.

SRY nuclear import is enhanced by hsc70 and CaM. Nuclear import of GFP-SRY-HMG-wild type (WT) and its mutant derivatives was reconstituted in vitro as per the legend to Fig. 1, with or without (No Add.) 1.5 μm hsc70 and/or CaM. CLSM images of cells at 18–20 min are shown (top panels), with TR70 indicating intact nuclei, for GFP-SRY-HMG-WT (A), the CaM-NLS (B), and β-NLS (C) mutant derivatives, with quantitative analysis of such images (bottom panels) as per the legend to Fig. 1. D and E, pooled data (mean ± S.E., n = 3) for percentage maximal nuclear accumulation (Fn/cmax) (D) and initial rate of nuclear accumulation (E) determined as per the legend to Fig. 1, in the presence and absence of the various additions for the indicated GFP-SRY-HMG derivatives. p values are shown where there were significant differences compared with in the absence of the addition of CaM and hsc70 proteins.