FIGURE 5.

Efficient nuclear import of SRY is dependent on hsc70 expression. A, representative CLSM images of the return of nuclear fluorescence after photobleaching in HeLa cells transfected to express full-length GFP-SRY- wild type (WT) in the presence or absence of siRNA to hsc70 over time (s) after photobleaching (post-bleach). Yellow dotted lines indicate regions of the nucleus photobleached. B, images such as those in A were quantified for the recovery over time of the nuclear to cytoplasmic fluorescence ratio (Fn/c) after photobleaching, expressed in terms of fractional recovery of respective time points divided by the initial (prebleach) value. Results shown are for a representative cell. C, pooled data for the mean ± S.E. (n > 13) for the percentage maximal recovery (left panel; normalized to 0 s post-bleach), time (s) taken to reach half maximal recovery (t½) (middle panel) and initial rate of recovery (right panel) in the presence and absence of siRNA to hsc70 for GFP-SRY-WT and GFP-T-ag NLS. p values are indicated where the values are significantly different in the presence of hsc70 siRNA.